Gene Set Enrichment Analysis Reveals That Fucoidan Induces Type I IFN Pathways in BMDC

Abstract Fucoidan, a sulfated polysaccharide extracted from brown seaweed, has been proposed to effectively treat and prevent various viral infections. However, the mechanisms behind its antiviral activity are not completely understood. We investigate here global transcriptional changes in bone marrow-derived dendritic cells (BMDCs) using RNA-Seq technology. Through both analysis of differentially expressed genes (DEG) gene set enrichment (GSEA), we found that fucoidan-treated BMDCs were enriched virus-specific response pathways, including SARS-CoV-2, as well pathways associated with nucleic acid-sensing receptors (RLR, TLR, NLR, STING), type I interferon (IFN) production. show these transcriptome driven by well-known regulators inflammatory against viruses, IRF, NF-κB, STAT family transcription factors. Furthermore, 435 950 upregulated DEGs classified IFN-stimulated (ISGs). Flow cytometric additionally showed fucoidan increased MHCII, CD80, CD40 surface markers BMDCs, indicative greater antigen presentation co-stimulation functionality. Our current study suggests transcriptionally activates PRR signaling, IFN production ISGs production, DC maturation, highlighting potential mechanism fucoidan-induced activity. 이 연구는 충남대학교 연구비와 교육부 조성 한국연구재단(NRF-2019R1I1A3A01060331 J.K.) 또한, 과학기술정보통신부가 지원하는 한국연구재단(NRF)을 통한 브레인풀 프로그램(NRF-2020H1D3A2A02048437, J.G.K.)과 과학기술부(NRF-2021M3H9A1030267, S.Y.K.)의 지원으로 진행됐다.